Uji Sitotoksisitas Ekstrak Metanol Alga Cokelat (Phaeophyta) Pada Lini Sel Kanker Payudara MCF - 7
Keywords:
brown algae, Phaeophyta, cytotoxic activity, anticancerAbstract
Marine algae (brown algae) have been considered as a source of bioactive compounds as they may be used as food, material for industry and herbal medicine. The objective of this study was to determine the cytotoxic activity of methanolic extracts of brown algae on breast cancer cell lines (MCF-7 cells) as anticancer candidates. Preperation of methanolic extract of brown algae have been done by using maseration method. The dry brown algae was extracted by using methanol, filtered, then dried. Cytotoxic activity test was conducted by using MTT assay on MCF-7 cells. The result showed that methanolic extract of Turbinaria ornata, T. deccurrens and Sargassum myriocystum had cytotoxic activity on MCF-7 cells with IC50 value of 221,88, 268,34 and 250,23 μg/ ml.Downloads
References
Aisa, Y., Miyakawa, Y., and Nakazato, T. (2005). Fucoidan induces apoptosis of human HS Sultan cell accompanied by activation caspase 3 and doen regulation of ERK pathway. American Journal of Hematology, 78 (1), 7-14
Athrikorala, Y., Jung, W.K., Vasanthan,T and Joen, Y.J. (2006). An anticoagulativepolysaccharide from an enzymatic hydrolysate of Eclonia cava. Arbohydrate Polymers, 66 (2), 184-191
Ayesha, Hira, V. Sultana, J. Ara, and S. Ehteshamul-Haque. (2010). In vitro cytotoxicity of seaweeds from Karachi Coast on brine shrimp. Pakistan Journal of Botany, 42(5), 3555-3560
Costa LS, Telles CBS, Oliveira RM, Nobre LTDB, Dantas-Santos N, Camara RBG, et al. (2011). Heterofucan from Sargassum filipendula induces apoptosis in HeLa Cells. Marine Drugs, 9, 603-614.
Croci DO, Cumashi A, Ushakova NA, Preobrazhenskaya ME, Piccoli A, Totani L., et al. (2011) Fucans, but not Fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from Laminaria saccharina Brown Seaweed. oPLoS ONE, 6(2), e17283
Ebada SS, Lin WH, and Proksch P. (2010). Bioactive Sesterterpenes and Triterpenes from Marine Sponges Occurrence and Pharmacological Significance. Marine Drugs. 8, 313-346.
Foley SA, Mulloy B, and Tuohy MG. (2011) An Unfractionated Fucoidan from Ascophyllum nodosum:Extraction, Characterization, and Apoptotic Effects in Vitro. Journal of Natural product, 74, 1851–1861.
Huang, H.L., Wu, S.L., Liao, H.F, Jiang, C.M., Huang, R.Y., Chen. Y.Y., Yang, Y.C., and Chen, Y.J. (2005). Induction of apoptosis by three marine algae through generation of reactive oxygen species in human leukemic cell lines. J. Agric. Food Chem, 53, 1776-1781
Kim, E.J, Park, S.Y., Lee, J., dan Park, J.H.Y.(2010): Fukoidan present in brown algae induces apoptosis of human colon cancer cells, Gastroenterology, 10,1-11
Kim, M., Ta, Q.V., Mendis, E., Rajapakse, N., Jung, W., Byun, H. Jeon, Y., dan Kim, S. (2006): Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity. Life Sci., 79, 1436.
Machana S, Weerapreeyakul, Barusrux, S, Nonpunya, A, Sripanidkulchai, B and Thitimetharoch, T. (2011). Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line. Chin.Med, 6, 39-46
Meiyanto, E., Susilowati., S., Tasminatun, S., Murwanti, R.,.dan Sugiyanto. (2007). Efek kemopreventif ekstrak etanolik Gynura procumbens (Lour), Merr pada karsinogenesis kanker payudara tikus Majalah Farmasi Indonesia, 18(3), 154 – 161, 2007
Meiyanto, E., Handayani, S., Septisetyani, E.P., dan Susidarti, R.A. (2009). Synergistic Effect of Areca catechu L. Ethanoloc Extract and its chloroform fraction with doxorubicin on MCF-7. Jurnal Ilmu Kefarmasian Indonesia, 7 (1), 13-18
Moosa, M.K. (1999). Sumberdaya laut nusantara, keanekaragaman hayati laut dan pelestariannya. Lokakarya Keanekaragaman Hayati Laut. Pemanfaatan secara lestari dilandasi penelitian dan penyelamatan. Widya Graha LIPI. Jakarta 23 Pebruari 1999
Pebriana, R.B., Wardhani, B.W.K., Widayanti, E., Wijayanti, N. L. S., Wijayanti, T. R., Riyanto, S dan Meiyanto, E. (2008). Pengaruh ekstrak metanolik daun kenikir (Cosmos caudatus Kunth.) trhadap pemacuan apoptosis sel kanker payudara. Pharmachon, 9 (1), 21-26
Pereira, DM, Cheel J, Areche C, San-Martin A, Rovirosa, J., Silva LR, Valentao, P., and Andrade, PB. (2011). Anti-Proliferative Activity of Meroditerpenoids Isolated from the Brown Alga Stypopodium flabelliforme against Several Cancer Cell Lines. Marine Drugs, 9, 852-862.
Regila, P., X Kazi,M.,Thyberg, J., Gaciong, Z., Swedenborg, J., and Hedin, U. (2000). Fucoidan inhibits smooth muscle cell proliferation and reduce mitogen activated protein kinase ability. European Journal of Vascular and Endovascular Surgery, 20, 419-426
Satoru, K., Noboru, T., Hiroo, N., Shinji, S., and Hiroshi, S. (2003). Oversulvation of fucoidan enhance its anti-angiogenic and antitumor activities. Bioch. Phar, 65, 173-179
Sheu, J.H.,. Wang, G.H., Sung P.J., and Duh, C.Y. (1999). New cytotoxic oxygenated fucosterol from the brown alga Turbinaria conoides. J. Nat. Prod., 62, 224-227
Sugawara, T., Matsubara, K., Akagi, R., Mori, M., dan Hirata, T. (2006): Antiangiogenic activity of brown algae fucoxanthin and its deacetylated product, Fucoxanthinol. J. Agric. Food Chem., 54, 9805−9810
Tang, H.F., Y. Yang-Hua, X.S. Yao, Q.Z. Xu, S.Y. Zhang and H.W. Lin. (2002). Bioactive steroids from the brown alga Sargassum carpophyllum. J. Asian Nat. Prod. Res., 4(2), 95-105.
Zandi, K., S. Tajbakhsh, I. Nabipour, Z. Rastian, F. Yousefi, S. Sharafian, dan K. Sartavi. (2010). In vitro antitumor activity of Gracilaria corticata (a red alga) against Jurkat and molt-4 human cancer cell lines. African Journal of Biotechnology, 9(40), 6787-6790
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