SELEKSI BAKTERI TERMOFILIK SELULOLITIK PASCA ERUPSI MERAPI

Authors

  • Anna Rakhmawati Jurusan Pendidikan Biologi, FMIPA, Universitas Negeri Yogyakarta
  • Evy Yulianti Jurusan Pendidikan Biologi, FMIPA, Universitas Negeri Yogyakarta
  • Eli Rohaeti Jurusan Pendidikan Kimia, FMIPA, Universitas Negeri Yogyakarta

DOI:

https://doi.org/10.14421/kaunia.1070

Keywords:

selection, bacteria, thermophilic, cellulolytic

Abstract

Thermophilic bacteria after the eruption of Merapi in 2010 have been isolated from the Gendol Atas river with dilution and enrichment methods. The purpose of this study was to conduct the selection of these bacteria as producers of cellulase enzymes. Selection was done by growing 348 bacterial isolates on selective media Mandels-CMC 0.5% using Carboxy Methyl Cellulose (CMC) as carbon source. Incubation was performed at 55 °C for 24 hours. The results showed 255 isolates (73.27%) were able to grow in Mandels medium-CMC 0.5%. Bacterial isolates were isolated by enrichment method (69.8%) were more able to grow on selective media compared with the dilution method (30.2%). The diameter of the three largest colonies were isolates D13a (2.44 cm); E135 (2.33 cm); and D110a (1.98 cm).

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Published

2014-10-31

How to Cite

Rakhmawati, A., Yulianti, E., & Rohaeti, E. (2014). SELEKSI BAKTERI TERMOFILIK SELULOLITIK PASCA ERUPSI MERAPI. Kaunia: Integration and Interconnection Islam and Science Journal, 10(2), 92–102. https://doi.org/10.14421/kaunia.1070

Issue

Vol. 10 No. 2 (2014)

Section

Articles

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