Bacterial Stock Culture (Pathogenic and Non-Pathogenic) Isolated from Eucheuma Spinosum in Marine Microbiology Laboratory/ Stok Kultur Bakteri (Patogen dan Non-Patogen) Diisolasi dari Rumput Laut Eucheuma spinosum di Laboratorium Mikrobiologi Laut
DOI:
https://doi.org/10.14421/edulab.2021.62.04Keywords:
Bacteri, Isolation, Non-Pathogen, Pathogen, SeaweedAbstract
The diversity and unique characteristics of bacteria in marine ecosystems are a fascinating topic to explore and utilize extensively. These bacteria are also often used to test in practicum and research activities in the Marine Microbiology laboratory. This study aims to isolate and identify pathogenic and non-pathogenic bacteria of Eucheuma Spinosum used for bacterial stock culture in the Marine Microbiology Laboratory. The isolation was carried out using the pour-plate technique. Colony morphology of bacteria was observed visually such as their shape, edge, elevation, and color. Identification was verified using VITEK MS tool and then, additional testing was conducted to clarify pathogenicity. This study found eight different bacterial species isolated from E. Spinosum after the pathogenic testing, six pathogenic bacteria were found such as Vibrio alginolyticus, Shewanella algae, Staphylococcus kloosii, Staphylococcus gallinarum, Pseudomonas stutzeri, and Staphylococcus arlettae. While the nonpathogenic bacteria indicating that they do not describe clinical symptoms are Bacillus cereus and Brevibacterium casei.
Abstrak
Keragaman dan keunikan dari bakteri yang berasal dari ekosistem laut sangat menarik minat peneliti untuk terus dikaji dan dimanfaatkan. Penggunaan bakteri ini juga sering dibutuhkan dalam kegiatan praktikum dan penelitian di laboratorium mikrobiologi laut sebagai bakteri uji. Penelitian ini bertujuan mengisolasi dan mengidentifikasi bakteri patogen dan non-patogen dari rumput laut Eucheuma spinosum (E. spinosum) yang akan dijadikan sebagai stok kultur bakteri di laboratorium mikrobiologi laut. Metode isolasi menggunakan metode agar cawan tuang, pengamatan morfologi koloni dilakukan secara visual bentuk, tepian, elevasi dan warna koloni bakteri. Identifikasi menggunakan alat VITEK MS dan selanjutnya dilakukan pengujian patogenisitas. Penelitian ini menghasilkan delapan spesies bakteri berbeda yang berhasil dipisahkan dari rumput laut E. Spinosum, setelah uji patogenisitas diperoleh enam isolat yang bersifat patogen yaitu bakteri Vibrio alginolyticus, shewanella algae, Staphylococcus kloosii, Staphylococcus gallinarum, Pseudomonas stutzeri dan Staphylococcus arlettae. Sedangkan bakteri yang tidak bersifat patogen dalam yang menunjukkan tidak memberikan gejala klinis pada rumput laut adalah Bacillus cereus dan Brevibacterium casei.
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References
Cappuccino, James G., and Natalie Sherman. Microbiology: A Laboratory Manual. Subsequent, Lab Manual edition. Menlo Park, Calif: Benjamin-Cummings Pub Co, 1998.
Chauhan, Prakram Singh, Bindi Goradia, and Bhavanath Jha. “Optimization and up Scaling of Ionic Liquid Tolerant and Thermo-Alkali Stable Laccase from a Marine Staphylococcus Arlettae S1-20 Using Tea Waste.” Journal of the Taiwan Institute of Chemical Engineers 86 (May 1, 2018): 1–8. https://doi.org/10.1016/j.jtice.2018.02.032.
DKP. Profil Rumput Laun Indonesia. Jakarta: Direktorat Jendral Perikanan Budidaya, 2004.
Dwidjoseputro, D. Dasar-Dasar Mikrobilogi. Jakarta: Djambatan, 1989.
Fu, Huihui, Peng Jiang, Jin Zhao, and Chunhui Wu. “Comparative Genomics of Pseudomonas Sp. Strain SI-3 Associated With Macroalga Ulva Prolifera, the Causative Species for Green Tide in the Yellow Sea.” Frontiers in Microbiology 9 (2018): 1458. https://doi.org/10.3389/fmicb.2018.01458.
Holt, H. M., B. Gahrn-Hansen, and B. Bruun. “Shewanella Algae and Shewanella Putrefaciens: Clinical and Microbiological Characteristics.” Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases 11, no. 5 (May 2005): 347–52. https://doi.org/10.1111/j.1469-0691.2005.01108.x.
Kamarudin, Kamarul Rahim, Nurziana Ngah, Tengku Haziyamin Tengku Abdul Hamid, and Deny Susanti. “Isolation of a Pigment-Producing Strain of Staphylococcus Kloosii from the Respiratory Tree of Holothuria (Mertensiothuria) Leucospilota () from Malaysian Waters.” Tropical Life Sciences Research 24, no. 1 (August 2013): 85–100.
Kunarso, Djoko Hadi. “Peranan Bakteri Heterotrofik dalam ekosistem Laut.” Oseana XIII, no. 4 (1988): 133–42.
Morfin-Otero, Rayo, Manuel A. Martínez-Vázquez, Daniel López, Eduardo Rodríguez-Noriega, and Elvira Garza-González. “Isolation of Rare Coagulase-Negative Isolates in Immunocompromised Patients: Staphylococcus Gallinarum, Staphylococcus Pettenkoferi and Staphylococcus Pasteuri.” Annals of Clinical & Laboratory Science 42, no. 2 (March 20, 2012): 182–85.
Nasution, M.H. Patogenitas Beberapa Isolat Bakteri Terhadap Rumput Laut Kappaphicus Alvarezii Asal Pulau Pari, Kepulauan Seribu. Jakarta: Fakultas Biologi Universitas Nasional Jakarta, 2005.
Pelczar, Michael J. Dasar-Dasar Mikrobiologi. universitas Indonesia Press, 1986.
Rahmani, Nanik, Ade Andriani, and Alex Prima. “Production and Characterization of Amylase Enzyme from Marine Bacteria,” n.d., 1.
Saraswati, Suprabadevi Ayumayasari, and I. Made Sena Darmasetiyawana. “Identifikasi Bakteri pada Rumput Laut Euchema spinosum yang terserang penyakit Ice-ice di Perairan Pantai Kutuh.” Journal of Marine and Aquatic Sciences 2, no. 1 (February 22, 2016): 11–15. https://doi.org/10.24843/jmas.2016.v2.i01.11-15.
Sidharta, Boy Rahardjo. Pengantar Mikrobiologi Kelautan. Yogyakarta: Universitas Atma Jaya Yogyakarta, 2000.
Singh, Ravindra Pal, and C.R.K. Reddy. “Seaweed–Microbial Interactions: Key Functions of Seaweed-Associated Bacteria.” FEMS Microbiology Ecology 88, no. 2 (April 1, 2014): 213–30. https://doi.org/10.1111/1574-6941.12297.
Yahya, Yahya, Happy Nursyam, Yenny Risjani, and Seomarno Soemarno. “Karakteristik Bakteri Di Perairan Mangrove Pesisir Kraton Pasuruan (Characterization of Bacteria Isolated from Mangrove Coastal Waters of Kraton, Pasuruan).” ILMU KELAUTAN: Indonesian Journal of Marine Sciences 19, no. 1 (March 3, 2014): 35–42. https://doi.org/10.14710/ik.ijms.19.1.35-42.
Zainuddin, Elmi Nurhaidah, Hilal Anshary, Huyyirnah Huyyirnah, Ridha Hiola, and Dolores V. Baxa. “Antibacterial Activity of Caulerpa Racemosa against Pathogenic Bacteria Promoting ‘Ice-Ice’ Disease in the Red Alga Gracilaria Verrucosa.” Journal of Applied Phycology 31, no. 5 (October 1, 2019): 3201–12. https://doi.org/10.1007/s10811-019-01805-w.
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